Garderobe Academy (ガルデローベ学園)
- Arika Yumemiya (アリカ・ユメミヤ?): A main character of My-Otome. She enters Garderobe dreaming of becoming an Otome like her mother. She possesses the Blue Sky Sapphire (蒼天の青玉 Souten no Seigyoku?), a memento from her mother. Voiced by: Mika Kikuchi (Japanese), Angie Beers (English)
- Nina Wáng (ニナ・ウォン Nina Won?): A main character of My-Otome. As the top Coral student, Nina dreams to become a great Otome to make Sergay, her adoptive father, proud. She dislikes Arika from the moment they meet although their relationship develops into friendship. Later in the series she receives the Ultimate Black Diamond (漆黒の金剛石 Shikkoku no Kongouseki?) and in My-Otome Zwei the Neptune Emerald (海神の翠玉 Watatsumi no Suigyoku?) as an interim contract with Mashiro. Voiced by: Ami Koshimizu (Japanese), Kris Rundle (English)
- Erstin Ho (エルスティン・ホー Erusutin Hō?): Arika's and Nina's roommate, from the country of Annan. Erstin mostly serves as a bridge between Arika and Nina, who don't get along very well. Voiced by: Minami Kuribayashi (Japanese), Lorie Barnes-Smith (English)
- Irina Woods (イリーナ・ウッズ Irīna Uzzu?): Irina is one of Arika's friends from the Aries Republic. She shows a love for technology and is under the tutelage of Yohko. Voiced by: Kumiko Higa (Japanese), Leah Dubbin-Steckel (English)
- Tomoe Marguerite (トモエ・マルグリット Tomoe Maruguritto?): She is Coral No. 2 and a room attendant for Chie. On the outside she acts normal but is jealous of Arika's and Shizuru's relationship. Later in the series she joins to the Valkyries (a system similar to Otome's although its power source is Lena Sayers' body instead of Fumi Himeno's) and becomes a somewhat leader figure in the forces. As a Valkyrie she wears the Cursed Obsidian (呪詛の黒曜石 Juso no Kokuyōseki?). Voiced by: Rie Tanaka (Japanese), Tracy Sutton (English)
- Lilie Adean (リリエ・アディーン Ririe Adīn?): Lilie is a member of Arika's class and one of Chie's room attendants. She, Miya and Yayoi are always hanging out together. She and Yayoi become honorary Maki Maki members by Shiho. She is possibly the My-Otome incarnation of My-HiME character Sayuri Shino. Voiced by: Akiko Kimura (Japanese), Jillian Hannah (English)
- Yayoi Alter (ヤヨイ・オールター Yayoi Ōrutā?): Another of Shiho's attendants and a girl with a very overactive imagination. She's from Lutetia Romulus. She and Lilie are made honorary Maki Maki members by Shiho. Voiced by: Kimiko Koyama
- Miya Clochette (ミーヤ・クロシェット Mīya Kuroshetto?): One of Shiho's room attendants. She is close friends with Tomoe. She’s from Lutesia Remus. Voiced by: Yui Itsuki (Japanese), Kelsey Schimpf (English)
- Akane Soir (アカネ・ソワール Akane Sowāru?): The top Pearl student and leader of the Trias. She becomes torn between her love for Kazuya and her dreams of becoming a Meister Otome (Ger. Jap. - literally 'Master Maiden'). The GEM she receives upon appointment as Meister is the Pure Heart Malachite (清恋の孔雀石 Seiren no Kujakuishi?). Voiced by: Junko Iwao (Japanese), Morgan Partridge (English)
- Chie Hallard (チエ・ハラード Chie Harādo?): Chie is Pearl number two and a member of the Trias. She is very boyish and often carries a blue rose around. Voiced by: Mitsuki Saiga (Japanese), Hazel Forman (English)
- Shiho Huit (シホ・ユイット Shiho Yuitto?): Shiho is Pearl number three and the last member of the Trias. She is from Cardair. She is usually seen conspiring to get revenge on everyone else by using a strange voodoo doll. The GEM she wears in episode 24 is the Spiral Spin Serpentine (螺旋の蛇紋石 Rasen no Jamonseki?). Voiced by: Sakura Nogawa (Japanese), Leda Davies (English)
- Juliet Nao Zhang (ジュリエット・ナオ・チャン Jurietto Nao Chan?): Pearl number four. She hails from Artai. She is a laid-back person, usually skipping classes and sneaking off of campus. However, she has been appointed as #4 of the Five Columns near the important part of the anime series, with the Break String Spinel (破絃の尖晶石 Hagen no Senshouseki?) as her GEM. Voiced by: Yuuka Nanri (Japanese), Marcy Lannan (English)
- Natsuki Kruger (ナツキ・クルーガー Natsuki Kurūgā?): The Headmistress of Garderobe and #2 of the Five Columns. She handles Garderobe's affairs and is the one who directly deals with the heads of state. Her GEM is the Ice Silver Crystal (氷雪の銀水晶 Hyōsetsu no Ginsuishō?). Voiced by: Saeko Chiba (Japanese), Cheryl McMaster (English)
- Shizuru Viola (シズル・ヴィオーラ Shizuru Viōra?): #3 of the Five Columns, known as the Bewitching Smile Amethyst (嬌嫣の紫水晶 Kyōen no Murasakisuishō?). She acts as an assistant (AKA Deputy Headmistress) to Natsuki. Voiced by: Naomi Shindō (Japanese), Melanie Risdon (English)
- Maria Graceburt (マリア・グレイスバート Maria Gureisubāto?): An inactive Meister Otome serving as part of Garderobe's upper administration. Strict and often-unsmiling, Miss Maria is feared by the students and even the other faculty members. She is from Old Lutesia and wears the Eternal Recurrence Jasper (久遠の碧玉 Kuon no Hekigyoku?). Voiced by: Yoko Matsuoka (Japanese), Barb Mitchell (English)
- Yukariko Steinberg (ユカリコ・シュタインベルグ Yukariko Shutainberugu?): The homeroom teacher for Nina and Arika as well as a Meister Otome. She is from Florince and wears the Dazzling Mirage Lapis Lazuli (幻惑の瑠璃 Genwaku no Ruri?). Voiced by: Kikuko Inoue (Japanese), Barbara Kozicki (English)
- Yohko Helene (ヨウコ・ヘレネ Yōko Herene?): Much like her My-HiME counterpart, Yohko Sagisawa, she's the Chief Medical Officer and the Director of Science Department of Garderobe, who is in charge of handling the technology that gives the Otome their powers. She has some past relationship with Aswad. Voiced by: Akiko Kimura
- Fumi Himeno (フミ・ヒメノ?): The first Otome who lived 300 years ago and possessed the ultimate GEM, the Pure White Diamond (真白なる金剛石 Mashiro naru Kongōuseki?). She is the source of power for the Otome at Garderobe.
- Sara Gallagher (サラ・ギャラガー Sara Gyaragā?): #1 of the Five Columns, wielder of the Galactic aquamarine (銀河の藍玉 Ginga no Rangyoku?). She first appears in episode 24, bringing gifts to Aries from Zipang. Her robe is batgirl-inspired. Her elements are giant boomerangs. Apart from that, she can make herself invisible. She has a habit of correcting Haruka's mispronunciations. She was also the room attendant of Tokiha Mai.Voiced by: Miyuki Sawashiro
- Mahya Blythe (マーヤ・ブライス Māya Buraisu?): #5 of the Five Columns, wielder of the Swirling Dance Fluorite (伶踊の蛍石 Reiyō no Hotaruishi?). She first appears in episode 24, arresting Akane after Kazuya was carried away by Cardair forces. She uses a pair of maracas to summon an immense creature composed of sand and earth that fights for her, shaking her maracas and dancing as a way to control the creature. Voiced by: Yumiko Kobayashi
- Elliot Chandler - During the days of Sifr Fran, Elliot was Column I and was the wielder of the Galactamarine. In the anime she is shown to be extremely attracted to Lena Sayers, going so far as to corner her in her hotel room and to try to have relations with her, which Lena resists.
- Kyoko Shimabuki - During the days of Sifr Fran, Kyoko was Column II and was the wielder of the Whirlwind Rose Quartz. Her element is a giant axe, with a pink and red robe. Not much else is known about her except that she went along with Una Shamrock's plans, and in the end was suspended from Otome duties for a month by Maria Graceburt at the conclusion of My Otome 0.
- Rei Zarganote - During the days of Sifr Fran, Rei was Column III and was the wielder of the Intensely Playing Peridot. Her element is a large sword, much like Mikoto's from My Hime, and her robe was orange and black. Not much else is known about her except that she went along with Una Shamrock's plans, and in the end was suspended from Otome duties for a month by Maria Graceburt at the conclusion of My Otome 0.
- Una Shamrock - During the days of Sifr Fran, Una was Column IV, Headmistress of Garderobe, and wielder of the Bewitching Smile Amethyst. She is set in eliminating Sifr, and even goes so far as to deauthorize Elliot as the Galactamarine to attack her. At the conclusion of My Otome 0, she is dismissed as Headmistress. It is unknown as to whether or not she remained a Column.
- Iruma Vandeveld - During the days of Sifr Fran, Iruma was Column V and wielder of the Ice Silver Crystal. Not much else is known about her except that she went along with Una Shamrock's plans, and in the end was suspended from Otome duties for a month by Maria Graceburt at the conclusion of My Otome 0.
Windbloom Kingdom (ヴィントブルーム王国)
- Mashiro Blan de Windbloom (マシロ・ブラン・ド・ヴィントブルーム Mashiro Buran Do Vintoburūmu?): The heir to the throne of Windbloom. She is initially very snobbish and childish, and is at odds with Arika, although it is heavily implied she is in love with Arika, specially in Mai Otome Zwei. Voiced by: Yukana (Japanese), Katie Rowan (English)
- Mikoto (ミコト?): A fat black cat owned by Mashiro.
- Sakomizu Cardinal (サコミズ・カージナル Sakomizu Kājinaru?): Captain of Windbloom's Royal Guards, constantly frustrated by Mashiro's willful antics. Voiced by: Akio Suyama (Japanese), Randall Wiebe (English)
- Aoi Senoh (アオイ・セノー Aoi Senō?): Mashiro's long-suffering maid who supports her despite her childish behaviors. She is in a relationship with Chie Hallard. An indicator that she swings that way could be the expression on her face at the end of the bath scene in the third episode of My-Otome Zwei (7:25). Voiced by: Ryoko Shintani (Japanese), Jayna Mitchell (English)
- Mimi (ミミ?): A young orphaned girl who lives in the slums of Windbloom. She hates Mashiro with a passion, a sentiment shared by many of Windbloom's poorer residents. Voiced by: Ai Shimizu
Artai Principality (アルタイ公国)
- Nagi Dài Artai (ナギ・ダイ・アルタイ Nagi Dai Arutai?): The Grand Duke of Artai. He appears to be manipulating political events. He also enjoys teasing Mashiro whom he has a love hate relationship with and tries to actually take control of her country. Voiced by: Akira Ishida (Japanese), Graham Ko (English)
- Sergay Wáng (セルゲイ・ウォン Serugei Won?): Commander in the Artai military and Nagi's right-hand man as well as Nina's adoptive father. Years ago, he fall in love of Arika's mother, Lena Sayers.Voiced by: Katsuyuki Konishi (Japanese), Phil Fulton (English)
- Tatsuhiko Gorvic Zaycech IV (タツヒコ・ゴロヴィッチ・ザイツェフⅣ世 Tatsuhiko Gorobitchi Zeishefu IV?): A student from Artai. Voiced by: Hisafumi Oda (Japanese), Jeffrey Watson (English)
- Archduke Bel Glan De Artai - He ruled Artai during the events depicted in the series, though he appears to have paid more attention to women than to affairs of state, amassing a total of six wives and children numbering into double figures. His death resulted in a power struggle which was only ended with the rise to power of Nagi Daí Artai, presumably his son. A throwaway comment by his successor suggests that many of his sons followed in Bel's footsteps, marrying poorly, causing Nagi to suggest that he should have ordered his amenuensis Sergay Wáng to assassinate them as well as their husbands.
It is possible Mashiro is his and Princess Sakura daughter, given the similarity with their
- Princess Sakura Hazakura - The Smoky Storm Chrysoberyl. A feisty Otome from the nation of Taiyun who serves Archduke Bel Glan De Artai during the time of Sifr Fran.
Aries Republic (エアリーズ共和国)
- Yukino Chrysant (ユキノ・クリサント Yukino Kurisanto?): The President of the Aries Republic and Haruka's master. She is assertive and portrays the image of a cool-minded leader. Voiced by: Mamiko Noto
- Haruka Armitage (ハルカ・アーミテージ Haruka Āmitēji?): A headstrong and impulsive Meister Otome who wears the Continental Orb Topaz (珠洲の黄玉 Suzu no Kōugyoku?). She has impressive strength and immediately takes a liking to Arika. She somehow believes that to be a good otome the person must have guts, which might be the reason why she does things without even thinking, sometimes including talking. Voiced by: Ryoka Yuzuki
- Midori (ミドリ?): The leader of Aswad. She has a serious personality, but gets along well with the people she leads. Voiced by: Yukari Tamura
- Rad (ラド Rado?): A cyborg who works under the command of Midori. He fights with a heavy double-ended spear. Voiced by: Toshihiko Seki (Japanese), Ethan Cole (English)
- Lumen (ルーメン Rūmen?): Another member of Midori's unit, who possesses a man's voice but a woman's body and speech pattern. Lumen fights with long metallic whips. Voiced by: Naoya Uchida
- Gal (ガル Garu?): A cyborg skilled with electronics who fights with a chakram, which usually floats above her head like a halo. She speaks using a combination of Japanese and English words. She has motorised skates on her feet and two electronic chords on her head(which can be used to access data). In Zwei, she is referred to as "professor". Voiced by: Kikuko Inoue
- Dyne (ダイン Dain?): A massive cyborg and part of Midori's team. He fights with a double-ended hammer. Voiced by: Eiji Miyashita
- Takumi Tokiha (鴇羽 巧海 頭忠頼 Tokiha Takumi no Kami Tadayori?): The son of the shogun of Zipang, traveling to Windbloom on a diplomatic mission. He turns out to be in search of Mai Tokiha from Garderobe, his sister who is lost after being torn apart between love and her future as an Otome years ago. Voiced by: Yugo Takahashi
- Akira Okuzaki (尾久崎 晶 Okuzaki Akira?): Akira is a kunoichi and Takumi's bodyguard. She tries to hid her identity as the king instead because Takumi went missing in Windbloom. Voiced by: Sanae Kobayashi
- Iori (伊織?): Takumi's attendant. Voiced by: Anri Katsu
Cardair Empire (カルデア帝国)
- Argos XIV (アルゴス14世 Arugosu 14?): One of the three emperors of Cardair, who hires Aswad secretly. Voiced by: Tomoaki Ikeda
- Fiar Grosse (フィア・グロス Fia Gurosu?): The Excel Elegance White Onyx (塊麗の縞瑪瑙 Kenrei no Shimamenō?), Argos XIV's Otome. She doesn't trust the Aswad, but she is very obedient to her master. Voiced by: Rie Tanaka
- Kazuya Krau-xeku (カズヤ・クラウゼク Kazuya Kurauzeku?): A second-year student at Windbloom University, and an heir to one of Cardair's three royal families. He is romantically involved with Akane. Voiced by: Kazuma Horie
- Charles Guinel Roy d'Florince VIII (シャルル・ギュネール・ロイ・デ・フロリンス八世 Sharuru Gyunēru Roi De Furorinsu 8?): The king of Florince. He seeks a new Otome to replace his retiring Otome, Rosalie. Voiced by: Mantarô Iwao
- Rosalie Claudel (ロザリー・クローデル Rozarī Kurōderu): The king of Florince's Otome, the Abyssal Green Jadeite (深淵の翡翠 Shin’en no Hisui?). She plans to retire in order to marry. In My Otome Zwei, she appears in the baths with the other girls and confronts Shiho Huit, expressing her dislike for the girl. Her element is a whip. Voiced by: Mikako Takahashi
United Kingdom of Lutesia (ルーテシア連合王国)
Lutesia Romulus (ルーテシア・ロムルス)
- The king of Lutesia Romulus
- Carla Bellini (カーラ・ベリーニ Kāra Berīni?): Lutesia Romulus' Otome, the Rumbling Thunder Garnet (雷鳴の柘榴石 Raimei no Zakuroishi?). Voiced by: Kiyomi Asai
Lutesia Remus (ルーテシア・レムス)
- The queen of Lutesia Remus
- Laula Bianchi (ラウラ・ビアンキ Raura Bianki?): Lutesia Remus' Otome, the Flowery Splendour Enstatite (絢爛の頑火輝石 Kenran no Gankakiseki?). Voiced by: Kikuko Inoue
An Nam (アンナン)
- Nguyễn Bảo (グエン・バオ Guen Bao?): The king of An Nam. He first appears in episode 21 on a visit to Aries. Voiced by: Eiji Miyashita
- Ảnh Lữ (アイン・ルー Ain Rū?): Nguyễn's Otome and daughter, the Infinite Wisdom Azurit (慧命の藍銅鉱 Emyō no Randōkō?). Famous for being unable to hold her alcohol. Her element is a double-ended halberd. Formerly she was Pearl #1. Voiced by: Ryoko Shintani
- John Smith (ジョン・スミス Jon Sumisu?): The blond-haired spokesman of Schwartz, an organization dedicated towards using the technology of the past for some as yet unstated goal. Voiced by: Naoya Uchida
- Mai Tokiha (鴇羽 舞衣 Tokiha Mai?): A Meister Otome who went missing, creating the legend of the "Tragic Otome". Her GEM is the Fire Stirring Ruby (炎綬の紅玉 Enju no Kougyoku?). Voiced by: Mai Nakahara (Japanese), Carol-Anne Day (English)
- Lena Sayers (レナ・セイヤーズ Rena Seiyāzu?): The former Meister Otome of the king of Windbloom. She vanished fourteen years ago during the attack on Windbloom Castle. Voiced by: Tomo Sakurai
- Miyu (ミユ?): A mysterious traveller who watches over Arika. Voiced by: Kiyomi Asai
- Alyssa (アリッサ Arissa?): Miyu's bird companion.
- Mikoto the Cat Goddess: A resident of the Black Valley. Voiced by: Ai Shimizu (Japanese), Caitlynne Medrek (English)
- Yamada (ヤマダ?): An information broker who works for Sergay and Nao. Voiced by: Hisanori Koyatsu
it:Personaggi di Mai-Otome pt:Mai-Otome zh:舞-乙HIME登場人物